MGB_SB Group

Our research is carried out in the Department of Biochemistry and Molecular Biology at UTMB. The laboratory is co-directed by Drs. Bradrick and Garcia-Blanco and is focused on two areas of biomedical importance: (i) host-pathogen interactions involving pathogenic flaviviruses such as dengue, yellow fever and zika viruses, and (ii) alternative splicing regulation of the interleukin-7 receptor alpha (IL7Rα) pre-mRNA and its connection to autoimmunity, especially in the context of multiple sclerosis.


People

Ruben Soto-Acosta

Education
  • School: CINVESTAV-Mexico
  • Major: Virology
  • Degree: Ph.D.
  • Year Graduated: 2015
  • School: Autonomous University of Chihuahua-Mexico
  • Major: Bacteriology
  • Degree: B.S.
  • Year Graduated: 2008
Bio & Experiences

I have experience working with dengue virus and herpes virus. As a postdoctoral fellow in Bradrick/Garcia-Blanco lab my research interest is to identify host cell factors involved in flavivirus infection in order to find potential therapeutic targets. Additionally, I am interested in the relation between subgenomic flavivirus RNA (sfRNA) and host cell proteins to identify mechanisms by which this subgenomic RNA is modulating the infectious process.

Publications

N.J. Barrows, R.K. Campos, S.T. Powell, K.R. Prasanth, G. Schott-Lerner, R. Soto-Acosta, G. Galarza-Munoz, E.L. McGrath, R. Urrabaz-Garza, J. Gao, P. Wu, R. Menon, G. Saade, I. Fernandez-Salas, S.L. Rossi, N. Vasilakis, A. Routh, S.S. Bradrick*, M.A. Garcia-Blanco* (2016). A screen of FDA-approved drugs for inhibitors of Zika virus infection. Cell Host & Microbe, doi: 10.1016/j.chom.2016.07.004. [Epub ahead of print] *corresponding authors

Soto-Acosta R, Mosso C, Cervantes-Salazar M, Puerta-Guardo H, Medina F, Favari L, Ludert JE, del Angel RM. The increase in cholesterol levels at early stages after dengue virus infection correlates with an augment in LDL particle uptake and HMG-CoA reductase activity. Virology.442:132-147.

Soto-Acosta R, Bautista-Carbajal P, Syed GH, Siddiqui A, Del Angel RM. 2014. Nordihydroguaiaretic acid (NDGA) inhibits replication and viral morphogenesis of dengue virus. Antiviral Res 109: 132-140.

Antonio Angel-Ambrocio**, Ruben Soto-Acosta**, Eshwar R Tammineni, Elba D. Carrillo, Patricia Bautista-Carbajal, Ascencion Hernandez, Jorge A. Sanchez and Rosa M. del Angel. An embryonic heart cell line is susceptible to Dengue Virus infection. Virus research 198: 53-58. (co-first author).

Margot Cervantes-Salazar, Antonio Angel-Ambrocio, Ruben SotoAcosta, Patricia Bautista-Carbajal, Juan Ludert, Rosa Maria del Angel The dengue virus non-structural protein 1 (NS1) interacts with the human ribosomal protein L18 in Huh-7 infected cells. Virology 484: 113-126.

Reyes-del Valle, J., Salas-Benito J., Soto-Acosta, R., and del Angel R.M. 2014. Dengue virus cellular receptors and tropism. Current Trop. Med. Reports. 1:36-43.

Gaddiel-2

Education
  • School: Duke University
  • Major: Molecular Genetics
  • Degree:Postdoctoral Fellow
  • Year Graduated: 2015
  • School: Institute of Neurobiology, University of Puerto Rico
  • Major: Biology (specialization in Molecular Neurobiology)
  • Degree: PhD
  • Year Graduated: 2012
Bio & Experiences

My research interests lie at the interface between genetic variation, RNA biology and disease: which and how genetic variations between individuals could affect gene expression leading to disease states. My current research at the Bradrick/Garcia-Blanco laboratory focuses on genetic variations influencing susceptibility to autoimmune diseases, in particular Multiple Sclerosis (MS). Previous research from our group uncovered a genetic variant affecting alternative splicing of the Interleukin-7 receptor (IL7R) as a risk factor for MS. I have extended this work to uncover the network of trans-acting factors controlling alternative splicing of IL7R, with the rationale that these represent candidate risk factors for MS. Validating this hypothesis, we identified the RNA helicase DDX39B as a potent activator of IL7R splicing and a novel risk factor for MS.

Awards and Honorable mentions

Recipient of NRSA F32 fellowship from the National Institute of Neurological Disorders and Strokes (NINDS).

Publications

Galarza-Muñoz G, Briggs FB, Evsyukova I, Schott-Lerner G, Kennedy EM, Nyanhete T, Wang L, Bergamaschi L, Widen SG, Tomaras GD, Ko DC, Bradrick SS, Barcellos LF, Gregory SG, Garcia-Blanco MA. Human Epistatic Interaction Controls IL7R Splicing and Increases Multiple Sclerosis Risk. Cell. 2017 Mar 23;169(1):72-84.e13. doi: 10.1016/j.cell.2017.03.007. PMID: 28340352

N.J. Barrows, R.K. Campos, S.T. Powell, K.R. Prasanth, G. Schott-Lerner, R. Soto-Acosta, G. Galarza-Munoz, E.L. McGrath, R. Urrabaz-Garza, J. Gao, P. Wu, R. Menon, G. Saade, I. Fernandez-Salas, S.L. Rossi, N. Vasilakis, A. Routh, S.S. Bradrick*, M.A. Garcia-Blanco* (2016). A screen of FDA-approved drugs for inhibitors of Zika virus infection. Cell Host & Microbe, doi: 10.1016/j.chom.2016.07.004. [Epub ahead of print] *corresponding authors

Galarza-Munoz et al., (2016). The RNA helicase DDX39B regulates alternative splicing of IL7R reducing the risk for Multiple Sclerosis. Under revision in Cell.

Geraldine

Education
  • School: University of Houston-Downtown
  • Major: Biology with Concentration in Microbiology
  • Degree:B.S.
  • Year Graduated: 2015
Bio & Experiences

I am interested in studying the mechanisms of subversion of host innate immune pathways by dengue and zika virus. I am currently studying the type III interferon response to infection and aim at investigating the mechanisms by which these two viruses turn off and/or overcome this immune response.

Publications

Galarza-Muñoz G, Briggs FB, Evsyukova I, Schott-Lerner G, Kennedy EM, Nyanhete T, Wang L, Bergamaschi L, Widen SG, Tomaras GD, Ko DC, Bradrick SS, Barcellos LF, Gregory SG, Garcia-Blanco MA. Human Epistatic Interaction Controls IL7R Splicing and Increases Multiple Sclerosis Risk. Cell. 2017 Mar 23;169(1):72-84.e13. doi: 10.1016/j.cell.2017.03.007. PMID: 28340352

N.J. Barrows, R.K. Campos, S.T. Powell, K.R. Prasanth, G. Schott-Lerner, R. Soto-Acosta, G. Galarza-Munoz, E.L. McGrath, R. Urrabaz-Garza, J. Gao, P. Wu, R. Menon, G. Saade, I. Fernandez-Salas, S.L. Rossi, N. Vasilakis, A. Routh, S.S. Bradrick*, M.A. Garcia-Blanco* (2016). A screen of FDA-approved drugs for inhibitors of Zika virus infection. Cell Host & Microbe, doi: 10.1016/j.chom.2016.07.004. [Epub ahead of print] *corresponding authors

Rafeal

Education
  • School: UFMG
  • Major: Microbiology
  • Degree: Master
  • Year Graduated: 2012
  • School: PUC-MG
  • Major: Biology
  • Degree: BS.
  • Year Graduated: 2011
Bio & Experiences

I am interested in how Flavivirus exploit the human host translation machinery. The genus Flavivirus comprises single-stranded RNA viruses, most of which are arthropod borne. Many of these viruses can cause important health impacts, and a few examples are Zika virus (ZIKV), Yellow Fever virus (YFV), and Dengue virus (DENV). While at the Garcia-Blanco Lab, I have the opportunity to study Flavivirus-Human interactions using state of the art techniques. I am utilizing data generated by our lab, with a genome-scale siRNA screen approach, to identify and further study host dependency factors for ZIKV, YFV and DENV.

Awards and Honorable Mentions
  • 2013 - Best Master's Thesis of Microbiology Department, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil
  • 2010 - Scientific Merit - Best Poster Awarded during International Symposium - Smallpox Eradication After 30 Years: Lessons, Legacies and Innovations. Sabin Vaccine Institute, Fogarty International Center of the U.S. National Institutes of Health, and Fundacao Oswaldo Cruz, 24 - 27 August. Rio de Janeiro, Brazil
Publications

Campos, R.K., Wong, B., Xie, X., Lu, Y.F., Shi, P.Y., Pompon, J., Garcia-Blanco, M.A., and Bradrick, S.S. (2017). RPLP1 and RPLP2 Are Essential Flavivirus Host Factors That Promote Early Viral Protein Accumulation. J Virol 91.

N.J. Barrows, R.K. Campos, S.T. Powell, K.R. Prasanth, G. Schott-Lerner, R. Soto-Acosta, G. Galarza-Munoz, E.L. McGrath, R. Urrabaz-Garza, J. Gao, P. Wu, R. Menon, G. Saade, I. Fernandez-Salas, S.L. Rossi, N. Vasilakis, A. Routh, S.S. Bradrick*, M.A. Garcia-Blanco* (2016). A screen of FDA-approved drugs for inhibitors of Zika virus infection. Cell Host & Microbe, doi: 10.1016/j.chom.2016.07.004. [Epub ahead of print] *corresponding authors

Campos, R.K., Boratto, P.V., Assis, F.L., Aguiar, E.R., Silva, L.C., Albarnaz, J.D., Dornas, F.P., Trindade, G.S., Ferreira, P.P., Marques, J.T., Robert, C., Raoult, D., Kroon, E.G., La Scola, B., Abrahao, J.S. Samba virus: a novel mimivirus from a giant rain forest, the Brazilian Amazon. (2014) Virol J. 11:95. doi: 10.1186/1743-422X-11-95.

Campos, R.K., Brum, M.C.S., Nogueira, C.E.W., Drumond, B.P., Alves, P.A., Siqueira-Lima, L.,Assis, F.L., Trindade, G.S., Bonjardim, C.A., Ferreira, P.C., Weiblen, R., Flores, E.F., Kroon, E.G., Abrahao, J.S. (2010). Assessing the variability of Brazilian Vaccinia virus isolates from a horse exanthematic lesion: coinfection with distinct viruses. Arch. Virol. 156:275-283.doi:10.1007/s00705-010-0857-z.

RPK

Education
  • School: National Chung-Hsing University, Taiwan
  • Major: Biotechnology
  • Degree: PhD
  • Year Graduated: 2011
Bio & Experiences

Worked as Postdoctoral fellow at Dept. of Plant pathology, University of Kentucky, USA . Majorly focussing on identification of host players in Tombusvirus replication and recombination and to unravel the mechanism.

Awards and Honorable mentions
  • Prasanth K.R and Nagy PD paper was selected as Highlighted manuscripts in Volume 489 by Virology, 2016.
  • MOE scholarship by Ministry of Education, Taiwan, for PhD studies
Publications

N.J. Barrows, R.K. Campos, S.T. Powell, K.R. Prasanth, G. Schott-Lerner, R. Soto-Acosta, G. Galarza-Munoz, E.L. McGrath, R. Urrabaz-Garza, J. Gao, P. Wu, R. Menon, G. Saade, I. Fernandez-Salas, S.L. Rossi, N. Vasilakis, A. Routh, S.S. Bradrick*, M.A. Garcia-Blanco* (2016). A screen of FDA-approved drugs for inhibitors of Zika virus infection. Cell Host & Microbe, doi: 10.1016/j.chom.2016.07.004. [Epub ahead of print] *corresponding authors

Muhammad Shah Nawaz-ul-Rehman#; K. Reddisiva Prasanth#; Kai Xu#; Zsuzsanna Sasvari #; Nikolay Kovalev; Isabel Fernandez de Castro Martin; daniel Barajas; Cristina Risco; Peter D. Nagy. Viral replication protein inhibits cellular cofilin actin depolymerization factor to regulate the actin network and promote viral replicase assembly. PLoS Pathogen (#Equal contribution).PLoS Pathog. 2016 Feb 10;12(2):e1005440.

K. Reddisiva Prasanth, Nikolay Kovalev, De castro Martin IF, Jannie Baker, Peter D. Nagy. Screening a yeast library of temperature sensitive mutants reveal a role for Actin in tombusvirus recombination. Virology. 2016 Feb;489:233-42.

K. Reddisiva Prasanth, Daniel Barajas, Peter D. Nagy. The proteasomal Rpn11 metalloprotease suppresses tombusvirus RNA recombination and promotes viral replication via facilitating the assembly of the viral replicase complex. Journal of Virology 2015 Mar;89(5):2750-63.

Chingkai Chuang, K. Reddisiva Prasanth, Peter D. Nagy. Coordinated function of cellular DEAD-box helicases in suppression of viral RNA recombination PLoS Pathogen. 2015 Feb 18;11(2):e1004680.

K. Reddisiva Prasanth, Ying-Wen Huang, Ming-Ru Liou, Robert Yung-Liang Wang, Chung-Chi Hu, Ching-Hsiu Tsai, Menghsiao Meng, Na-Sheng Lin, and Yau-Heiu Hsu. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) negatively regulates the replication of Bamboo mosaic virus and its associated satellite RNA. Journal of Virology, Sept. 2011, p. 8829-8840.

Nawaz-ul-Rehman MS, Reddisiva Prasanth K, Baker J, Nagy PD. Yeast screens for host factors in positive-strand RNA virus replication based on a library of temperature-sensitive mutants. Methods. 2013 Feb; 59(2):207-16.

Priya Balakrishnan#, K. Reddi Sivaprasanth#, V. Dhivya Jensi, L. Uma, G. Subramanian and D. Prabaharan. (2010) Characterization of manganese superoxide dismutase gene from a marine cyanobacterium Leptolyngbya valderiana BDU20041. Saline systems 6: 6 (#Equal contribution).

Chad

Education
  • School: Duke University- Mascot Branch
  • Major: General Mascoting
  • Degree: PhD
  • Year Graduated: 2014
  • School: Mascot University
  • Major: Communications
  • Degree: B.S.
  • Year Graduated: 2007
Awards and Honorable mentions
  • MGB Lab- Best Mascot-2016
  • Mascot of the Year-2015
  • Best Wingman - 2015
Victoria

Education
  • School: Universidad Central de Venezuela
  • Major: Medicine
  • Degree: M.D.
  • Year Graduated: 2016
Bio & Experiences

Born in Caracas-Venezuela She received her Medical degree at Universidad Central de Venezuela on December 2016. Dr. Medina Carbonell participated in the development of research of Pharmacological modulation of pulmonary embolism at the Section of Respiratory Physiology and then on 2014 at the Section of Celular Biology in research on characterization of the proteomic and histologic profile of liver tissue treated with Doxorrubycin with and without L-Carnitin supplementation.

Awards and Honorable mentions

First place at Scientific Review Contest: Huntington disease review. Review. III International Scientific Congress of Heath Science Students. XXI Century Diseases.
Outstanding Performance in Scientific Literature Activity, academic year 2011-2012 , Contribution: "Flow mediated dilation , a current technique Review". Department of Physiology. School Luis Razetti. UCV.

Publications

Dager A, Medina-Carbonel V, Vargas L, Moreno D. EFECTOS DEL ÁCIDO ACETIL SALICÍLICO EN UN MODELO DE TROMEMBOLISMO PULMONAR AGUDO. Pulmón. 2014;4(8):13 - 20


Previous Lab Personnel
  • Nicholas J. Barrows - Post Doc at St. Judes
  • Steven Powell - Student at
  • Shoko Nakamura