- School: Hokkaido University
- Major: Veterinary Science
- Degree: Ph.D.
- Year Graduated: 2018
- School: Hokkaido University
- Major: Veterinary Medicine
- Degree: D.V.M.
- Year Graduated: 2014
Awards and Honorable mentions
- Oversea Postdoctoral Fellowship, Uehara Memorial Foundation (2018-Present)
- Research Fellowship for Young Scientists (DC-1), Japan Society for the Promotion of Science (2015-2018)
- Scholarship, One Health Leading Graduate School program, Hokkaido University (2014-2015)
Bio & Experiences
My research interest is molecular pathology of disease caused by disruption of RNA and RNA-binding Protein interaction. During my graduate education, I have experience working with neurotropic flaviviruses, including Japanese encephalitis virus, West Nile virus, and Tick-borne encephalitis virus. As a postdoctoral fellow in Bradrick/Garcia-Blanco lab Currently, I am working on the projects of alternative splicing on immune-dysfunction in multiple sclerosis and tumor.
M Nakayasu, M Hirano, M Muto, S Kobayashi, H Kariwa, K Yoshii*. Development of a serodiagnostic IgM-ELISA against tick-borne encephalitis virus using subviral particles with strep-tag (2018) Ticks Tick Borne Dis., 9(6):1391-1394. doi: 10.1016/j.ttbdis.2018.06.010.
M Muto, W Kamitani, M Sakai, M Hirano, S Kobayashi, H Kariwa, K Yoshii*. Identification and analysis of host proteins that interact with the 3’ untranslated region of tick-borne encephalitis virus genomic RNA. (2018) Virus Res. 249:52-56. doi: 10.1016/j.virusres.2018.03.006.
M Hirano, M Muto, M Sakai, H Kondo, S Kobayashi, H Kariwa, K Yoshii*. Dendritic transport of tick-borne flavivirus RNA by neuronal granules affects development of neurological disease. (2017) Proc Natl Acad Sci USA. 114(37):9960-9965. doi: 10.1073/pnas.1704454114
L Eyer, H Kondo, D Zouharova, M Hirano, JJ Valdés, M Muto, T Kastl, S Kobayashi, J Haviernik, M Igarashi, H Kariwa, M Vaculovicova, J Cerny, R Kizek, A Kröger, S Lienenklaus, M Dejmek, R Nencka, M Palus, J Salat,E De Clercq, K Yoshii, D Ruzek*. Escape of tick-borne flavivirus from 2′-C-methylated nucleoside antivirals is mediated by a single conservative mutation in NS5 that has a dramatic effect on viral fitness. (2017) J Virol. 91:e01028-17. doi: 10.1128/JVI.01028-17.
S Kobayashi*, K Yoshii, M Hirano, M Muto, H Kariwa. A novel reverse genetics system for production of infectious West Nile virus using homologous recombination in mammalian cells. (2017) J Virol Methods. 2017;240:14-20. doi:10.1016/j.jviromet.2016.11.006.
E Inagaki, M Sakai, M Hirano, M Muto, S Kobayashi, H Kariwa, K Yoshii*. Development of a serodiagnostic multi-species ELISA against tick-borne encephalitis virus using subviral particles. (2016) Ticks Tick Borne Dis. 2016;7(5):723-729. doi:10.1016/j.ttbdis.2016.03.002.
M Sakai, M Muto, M Hirano, H Kariwa, K Yoshii*. Virulence of tick-borne encephalitis virus is associated with intact conformational viral RNA structures in the variable region of the 3′-UTR. Virus Res. 2015;203:36-40. doi:10.1016/j.virusres.2015.03.006.
M Hirano, K Yoshii*, M Sakai, R Hasebe, O Ichii, H Kariwa. Tick-borne flaviviruses alter membrane structure and replicate in dendrites of primary mouse neuronal cultures. (2014) J Gen Virol. 2014;95(Pt_4):849-861. doi:10.1099/vir.0.061432-0.
M Sakai, K Yoshii*, Y Sunden, K Yokozawa, M Hirano, H Kariwa. Variable region of the 3’ UTR is a critical virulence factor in the Far-Eastern subtype of tick-borne encephalitis virus in a mouse model. (2014) J Gen Virol. 2014;95(Pt_4):823-835. doi:10.1099/vir.0.060046-0.